convenient way to merge microscope images

1029 views5 repliesLast post: 7/15/2006
I'm a summer student at aresearch lab. I am using the Zeiss Axiovert S100
( http://kugrserver.health.unm.edu:16080/microscopy/_images/In s3.jpg) inverted microscope and the Zeiss Axiocam to take pictures of brain tissue at 10x magnification. These are 1312x1030 pixel pictures. The images were taken from left to right sequentially. So the whole image is a grid of smaller images that has 40 rows with 8 images per row. I would like to combine all of these pictures by merging their overlapping regions. Is there a convenient way of doing this with any stitching programs? Can MatLab do this? I'm looking for freeware or trial versions. I do have Photoshop CS.
Thanks
#1
wrote in message
I'm a summer student at aresearch lab. I am using the Zeiss Axiovert S100
( http://kugrserver.health.unm.edu:16080/microscopy/_images/In s3.jpg) inverted microscope and the Zeiss Axiocam to take pictures of brain tissue at 10x magnification. These are 1312x1030 pixel pictures. The images were taken from left to right sequentially. So the whole image is a grid of smaller images that has 40 rows with 8 images per row. I would like to combine all of these pictures by merging their overlapping regions. Is there a convenient way of doing this with any stitching programs? Can MatLab do this? I'm looking for freeware or trial versions. I do have Photoshop CS.
Thanks

try the photo merge feature in CS
it is under file>automate>
#2
wrote in message
I'm a summer student at aresearch lab. I am using the Zeiss Axiovert S100
( http://kugrserver.health.unm.edu:16080/microscopy/_images/In s3.jpg) inverted microscope and the Zeiss Axiocam to take pictures of brain tissue at 10x magnification. These are 1312x1030 pixel pictures. The images were taken from left to right sequentially. So the whole image is a grid of smaller images that has 40 rows with 8 images per row. I would like to combine all of these pictures by merging their overlapping regions. Is there a convenient way of doing this with any stitching programs? Can MatLab do this? I'm looking for freeware or trial versions. I do have Photoshop CS.
Thanks

sorry didn't realize how many images, it may only work to make panoramas with up to 6 images
#3
Try Panavue, I think they have a trial version.

http://www.panavue.com/en/gallery/panavue/microscope.htm

Bruce

wrote in message
I'm a summer student at aresearch lab. I am using the Zeiss Axiovert S100
( http://kugrserver.health.unm.edu:16080/microscopy/_images/In s3.jpg) inverted microscope and the Zeiss Axiocam to take pictures of brain tissue at 10x magnification. These are 1312x1030 pixel pictures. The images were taken from left to right sequentially. So the whole image is a grid of smaller images that has 40 rows with 8 images per row. I would like to combine all of these pictures by merging their overlapping regions. Is there a convenient way of doing this with any stitching programs? Can MatLab do this? I'm looking for freeware or trial versions. I do have Photoshop CS.
Thanks
#4
On Wed, 12 Jul 2006 16:32:00 -0400, "KatWoman" wrote:

wrote in message
I'm a summer student at aresearch lab. I am using the Zeiss Axiovert S100
( http://kugrserver.health.unm.edu:16080/microscopy/_images/In s3.jpg) inverted microscope and the Zeiss Axiocam to take pictures of brain tissue at 10x magnification. These are 1312x1030 pixel pictures. The images were taken from left to right sequentially. So the whole image is a grid of smaller images that has 40 rows with 8 images per row. I would like to combine all of these pictures by merging their overlapping regions. Is there a convenient way of doing this with any stitching programs? Can MatLab do this? I'm looking for freeware or trial versions. I do have Photoshop CS.
Thanks

try the photo merge feature in CS
it is under file>automate>
I hate to mention another program in this group :-)
but I find PanaVue image assembler to be ideal

Keith J (BoilerBill) Chesworth
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#5
wrote:

I'm a summer student at aresearch lab. I am using the Zeiss Axiovert S100
( http://kugrserver.health.unm.edu:16080/microscopy/_images/In s3.jpg) inverted microscope and the Zeiss Axiocam to take pictures of brain tissue at 10x magnification. These are 1312x1030 pixel pictures. The images were taken from left to right sequentially. So the whole image is a grid of smaller images that has 40 rows with 8 images per row. I would like to combine all of these pictures by merging their overlapping regions. Is there a convenient way of doing this with any stitching programs? Can MatLab do this? I'm looking for freeware or trial versions. I do have Photoshop CS.
Thanks

There's a piece of software called enblend that can do this. Another called autopano. You give them a series of images and the software figures out how they fit together and then does it.

These are command line utilities written for linux, but they're open source and so you could find the source and compile it for whatever platform you need, or you could find a binary for your platform.

Really, though, it seems like Zeiss would have a solution for this.
#6