NEWBIE HELP: multiple images in one file

RH
Posted By
r.hartley
Sep 12, 2003
Views
350
Replies
2
Status
Closed
Hi All.

I have a laser microscope which takes accurate slices through tissue and displays as a series of z-sections. I can open the file in photoshop using the biorad confocal import filter. However, the import filter takes the one 3D file and converts it to eg. 30 single images. I want to correct the chromatic abberatuion in the files (due to the different wavelengths of each laser, there are 4 in the microscope.) This would be easier if there was a method of encapsulating all the images into one pseudo-movie file. I have found a good filter for this and it is only 200dollars

Is there a way to display the images in one file,

This is a bit like opening 30 images in photoshop and you see them all stacked one on top of each other. They are individual files though. I would like to have all the images in the one file and have only the first visible and then scroll throught the images as if you were flicking through a book. If you knwo what I mean.

Cheers
bob
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AO
Anguished_Oink
Sep 13, 2003
Dont think you can easily do what your after in PS from what you have discribed.

I would like to have all the images in the one file and have only the first visible and then scroll throught the images as if you were flicking through a book

The closest thing to this would be having an image with 30 layers each layer being one of your single images.
It would be a pain to have to create this every time you work on a new file unless you created a batch or droplet to open each image and cut/paste on new canvas to easily get your file with 30 layers( Just a thought).

Have you looked to see if there is some software that would handle these ‘biorad confocal’ files better than photoshop?
Mabye check-out http://www.biomed2.man.ac.uk/ireland/confocal_software.htm

Gook Luck!
Cheers

"Bob Hartley" wrote in message
Hi All.

I have a laser microscope which takes accurate slices through tissue and displays as a series of z-sections. I can open the file in photoshop using the biorad confocal import filter. However, the import filter takes the one 3D file and converts it to eg. 30 single images. I want to correct the chromatic abberatuion in the files (due to the different wavelengths of each laser, there are 4 in the microscope.) This would be easier if there was a method of encapsulating all the images into one pseudo-movie file. I have found a good filter for this and it is only 200dollars

Is there a way to display the images in one file,

This is a bit like opening 30 images in photoshop and you see them all stacked one on top of each other. They are individual files though. I would like to have all the images in the one file and have only the first visible and then scroll throught the images as if you were flicking through a book. If you knwo what I mean.

Cheers
bob
RH
r.hartley
Sep 17, 2003
"Anguished_Oink" …
Hi there

Have you looked to see if there is some software that would handle these ‘biorad confocal’ files better than photoshop?
Mabye check-out http://www.biomed2.man.ac.uk/ireland/confocal_software.htm

I actually have some top apps on my machine that reads the files.

Metamorph http://www.universal-imaging.com/products/metamorph/ Neurolucida www.microbrghtfield.com
AMIRA www.tgs.com

I also have the freebie ImageJ and Confocal assistant and Imagetool.

All are great for things but they cannot correct the (non-centred) abberation. In addition I use PS for montqgeing and the fileter for correction is great for non-planar warping (fisheye)

Looks like I’ll have to get my mate to write a plugin for all.

Where do you get the PS SDK these days? I cant find it.

cheers
Bob

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